Pyrosequencing as implemented by Roche's 454 is a technology that generates a large number of intermediate length DNA reads through a massively parallel sequencing-by-synthesis approach 1. The GS FLX ...

Figure 1: Enzymatic cascade of the pyrosequencing reaction in the example of a bisulfite-treated template sequence, including a CpG position that is methylated on approximately 50% of all molecules.

Multiple fields of microbiology rely on techniques of microbial identification, such as in food microbiology for detecting contaminants, and in microbial ecology to characterize biodiversity. Image ...