Brianna Bibel on Instagram: "Histidine biochemistry and His-tag chromatography. HISTIDINE (His, H) may be an amino *acid* but it can also be a base (or a “double” acid)! When it comes to protons (H⁺), it’s a game of give & take! And His’ metal-binding ability can be used to make nickel-linked little beads “give and take” “His-tagged” proteins to help you purify them in Immobilized Metal Affinity Chromatography (IMAC). https://youtube.com/shorts/O0aMzw7J7yc?feature=share blog form: http://bit.ly/