Steps by Steps Tissue Processing Fixation Dehydration embedding Histopathology
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In histopathology, cutting of frozen sections is a rapid tissue processing technique mainly used for intraoperative diagnosis (to guide surgeons during surgery). Instead of the usual paraffin embedding (which takes hours to days), the frozen section method allows diagnosis within minutes. Here’s a breakdown: --- Principle Fresh tissue is rapidly frozen to preserve its structure. The frozen tissue becomes firm enough to be cut into very thin sections using a special microtome (cryostat). Sections
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