The Excel sheet used to create exemplary figures (“Example qPCR”) as well as the Excel sheet that compares the regular 2-ΔΔCT method to the adjusted version ...

qPCR is a technique for the selective amplification and quantitative detection of regions of DNA or complimentary DNA (cDNA). Oligonucleotide primers flanking a region of interest are used to amplify ...

RT-qPCR stands for quantitative reverse transcription polymerase chain reaction, the “RT” not to be mistaken for “real time”. 2 Unlike qPCR that uses a DNA template, the starting material for RT-qPCR ...

When working in a lab, launching a quantitative PCR (qPCR) experiment demands a comprehensive understanding of complicated protocols, principles, and methodologies. Analyzing the large amounts of ...

Background: the gold-standard diagnostic protocol (GSDP) for COVID-19 consists of a nasopharyngeal swab (NPS) sample processed through traditional RNA extraction (TRE) and amplified with ...

Reliable extraction and sensitive detection of RNA from human peripheral blood mononuclear cells (PBMCs) is critical for a broad spectrum of immunology research and clinical diagnostics. RNA analysis ...

BEVERLY, Mass.--(BUSINESS WIRE)--Quantabio, a leading provider of robust DNA and RNA amplification reagents for the most demanding molecular testing and life science research applications, today ...

Large numbers of drug discovery projects involve the analysis of gene-expression levels to accurately assess target effects. When screening compounds from a library, it is common to use microarray ...

Creative Commons (CC): This is a Creative Commons license. Attribution (BY): Credit must be given to the creator. Emerging and evolving Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) ...