Oncolytic adenovirus is one of the most promising treatments against cancer and is widely evaluated clinically. During high titer production, "Wild-type-" like replication-competent adenovirus (RCA) ...
1 Key Laboratory of Biotoxin Analysis and Assessment for State Market Regulation, Nanjing Institute of Product Quality Inspection, Nanjing, China 2 School of Food Science and Pharmaceutical ...
Chinese hamster ovary (CHO) cells are the host cell of choice for manufacturing biologic drugs, like monoclonal antibody, in the biopharmaceutical industry. Retrovirus-like particles (RVLPs) are made ...
A new assay method for natural killer (NK) cell activity was established using quantitative RT-PCR (RT-qPCR) to determine the gene expression of granzyme B (GzmB) and perforin 1 (Prf1). The RT-qPCR ...
1 Exotic Herbivore Disease Surveillance and Research Center, China Animal Health and Epidemiology Center, Qingdao, China 2 College of Veterinary Science, Qingdao Agricultural University, Qingdao, ...
Real-time polymerase chain reaction reverse transcription (RT-qPCR) has been described as the gold standard for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) detection. While the ...
These R scripts take raw Cq data from qPCR runs, clean and average the triplicate data, and then perform the ddCt method as per Livak and Schmittgen (2001). The cq data can be downloaded from the ...
1 Key Laboratory of Biotechnology and Bioresources Utilization of Ministry of Education, Dalian Minzu University, Dalian, China. 2 Dalian Polytechnic University, Dalian, China. 3 Beijing Animal ...
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