Gene therapy has often been described as a “black box” technique; that is, it is not known what happens to the cell after vector-mediated gene transfer. Although expression levels of the transgene are ...

Figure 1: cDNA microarray analysis of DNA copy-number changes. Figure 3: Genome-wide mapping of DNA copy-number variation for breast cancer cell line BT474. Figure 4: High-resolution analysis of ...

Serial analysis of gene expression (SAGE) uses mRNA from a sample to create complementary DNA (cDNA) fragments for amplification and sequencing using high-throughput sequencing technology. Credit: ...

To identify differentially expressed genes and scan candidate molecular markers in nasopharyngeal carcinoma (NPC). We constructed four subtracted cDNA libraries using suppression subtractive ...

Technology Set to Compete with Microarrays in This Application Arena Microarray-based gene-expression profiling technology hardly seems like it has been around long enough for a new approach to come ...

Microarray technology has burgeoned over the past few years from nucleic acid-based arrays to tissue microarrays (TMAs). This study aimed to develop a technique to incorporate cell lines into an array ...

A microarray is a two-dimensional arrangement of biological samples allowing for high-throughput analysis of genetic information. DNA microarrays are commonly used to genotype multiple regions of a ...

Current microarray analysis uses 'chips' containing either 25-residue oligonucleotides synthesized by photolithography or cDNAs placed by robotic spotting. In the April Nature Biotechnology, Hughes et ...