Figure 1: Schematic representation of the continuous FFE apparatus coupled off-line to RP-HPLC. Hydroxypropyl methyl cellulose (HPMC) stock solution, 2% (wt/vol; ∼86 K nominal molecular weight; ...

High-performance liquid chromatography (HPLC) is an analytical chemistry technique used to separate liquid mixtures of compounds or analytes. The method employs a liquid "mobile phase" to transport ...

A main goal of any U/HPLC analysis is to achieve the optimum sensitivity with the given instrumentation. Here are a few simple measures that can be easily implemented. A main goal of any U/HPLC ...

Modern HPLC method development is dominated by a small number of pH adjusting reagents and buffers that are prevalent even when the method uses UV detection. This is driven primarily by the ...

This article presents a simple and systematic approach to HPLC method development, beginning with sample preparation and finishing with practical analytical method validation. step 5 - method ...

Metabolite profiling plays a crucial role in drug discovery and development, and HPLC-Q-TOF has evolved into a powerful and effective high-resolution analytical tool for metabolite detection. However, ...

There are a few scenarios where analytical procedures might need to be transferred between HPLC instruments: 1) Method transfer to new equipment in the same lab, perhaps to a different vendor or a ...

When conducting reproducible and interference-free analysis by liquid chromatography, the purity of the solvents used, particularly the purity of water, is crucial for the sensitivity of this method, ...

Radio-HPLC is a powerful tool for analyzing radioactive species in radiopharmaceutical chemistry. In this paper, we found an example that the commonly used eluting solvent, acetonitrile, could ...

Investigation of peak purity failure during HPLC method validation led to discovery of a co-eluting impurity under the main peak. Spectral analysis, including three-dimensional modelling, was used to ...