According to proteomics analyses, more than 70 different ion channels and transporters are harbored in membranes of intracellular compartments such as endosomes and lysosomes. Malfunctioning of these ...

Understanding non-stationary neuronal activity as seen in vivo requires estimation of both excitatory and inhibitory synaptic conductances from a single trial of recording. For this purpose, we ...

Abstract: The patch clamp technique has been widely utilized to measure extremely weak ion channel signals. In patch clamp technique, the measurement accuracy and duration rely on the tightness and ...

The dynamic clamp method was introduced independently by Robinson and Kawai 1 and Sharp et al. 2 Initially it was used to introduce artificial conductances into neurons to mimic the effect of ...

The whole-cell patch-clamp method is a gold standard for single-cell analysis of electrical activity, cellular morphology, and gene expression. Prior to our discovery that patch-clamp pipettes could ...

It is important to note that Bédard and colleagues have also described a method for the single trial extraction of synaptic input (Bédard et al., 2012). This method, which requires that the membrane ...

Dr. Kaneez Fatima Shad knows that the “Patch Clamp” method needs skills and patience. It takes the whole day to craft only one sample and even a small leak could alter the results drastically.