Next-generation sequencing requires intact and high-quality DNA. However, typical liquid-nitrogen DNA extraction methods are expensive and not practical for field sample collections. Hence, we present ...

Figure 3: Construction of lesion-bearing plasmids. Compared with conventional transcriptional lesion bypass and mutagenesis assays, our CTAB method is more efficient ...

Citrus species are recalcitrant to DNA extraction due to high levels of polysaccharides, polyphenols, and secondary metabolites that interfere with DNA yield and purity. This study presents an ...

The following protocols can be used for homogenizing the solutions: 650, 700, and 750 nm wavelengths: A vial filled with GNRs is placed in a warm water bath of 30 to 35°C for around 15 minutes; the ...

Introduction: The increasing global pressure to explore alternative protein sources derived from animal by-products has opened-up opportunities, but it has also created the need to assess their ...

ABSTRACT: Transgenic food safety is a high-profile public health issue in worldwide, especially transgenic soybean (Glycine max L.) oil. To rapidly and effectively detect transgenic components of ...

In this work, the surfactant cetyltrimethylammonium bromide (CTAB) was applied to observe how increasing the surfactant's concentration will affect the MgO characteristics, such as the increase in ...