* To clean pestels (eppendorf-size pestel), wash well, rinse with 100% ETOH and with distilled water. Then autoclave them to avoid contamination. * Label 3 sets of 1.5 ml eppendorf tubes. A maximum of ...

* All reagents and chemicals used for RNA extractions should never be used for other purposes. * Always manipulate everything with clean nitrile gloves. ## Tissue grinding * Use super clean mortar and ...

Ninety-one taxa were subjected to RNA extraction with three methods presented here: (1) TRIzol/TURBO DNA-free kits using the manufacturer's protocol with the addition of sarkosyl; (2) a combination ...

Cetyltrimethylammonium bromide (CTAB)-based methods are widely used to isolate DNA from plant tissues, but the unique chemical composition of secondary metabolites among plant species has necessitated ...

Typically, DNA is extracted from a plant sample using a method called CTAB extraction, which has to be done in a lab, requires a lot of equipment, and takes at least 3 to 4 hours. CTAB extraction is a ...